Antimicrobial effects of ozonated water on the sanitization of dental instruments contaminated with E. coli, S. aureus, C. albicans, or the spores of B. atrophaeus

Abstract

Objectives: Ozone has been used as an alternative method for the decontamination of water, food, equipment and instruments. The objective of this study was to evaluate the antimicrobial effects of ozonated water on the sanitization of dental instruments that were contaminated by Escherichia coli, Staphylococcus aureus, Candida albicans and the spores of Bacillus atrophaeus. Methods: A total of one hundred and twenty standardized samples of diamond dental burs were experimentally contaminated with E. coli (ATCC 25922), S. aureus (ATCC 6538) and C. albicans (ATCC 18804) and the spores of B. atrophaeus (ATCC 6633) for 30min. After the contamination, the samples were exposed to ozonated water (10mg/L O3) for 10 or 30min. The control group was composed of samples that were exposed to distilled water for 30min. After the exposure to the ozonated water, 0.1mL aliquots were seeded onto BHI agar to count the colony-forming units per milliliter (CFU/mL) of E. coli, S. aureus, and B. atrophaeus. Sabouraud dextrose agar was used to count the CFU/mL of C. albicans. The results were subjected to an analysis of variance and the Tukey test. Results: For all of the microorganisms studied, the ozonated water reduced the number of CFU/mL after 10 and 30. min of sanitization, and this microbial reduction was dependent on the duration of the exposure to the ozonated water. E. coli exhibited the greatest reduction in CFU/mL (2.72-3.78. log) followed by S. aureus (2.14-3.19. log), C. albicans (1.44-2.14. log) and the spores of B. atrophaeus (1.01-1.98. log). Conclusion: The ozonated water was effective in reducing the CFU of E. coli, S. aureus, C. albicans and B. atrophaeus spores, suggesting that ozonated water can be used for the sanitization of dental instruments. © 2012 King Saud Bin Abdulaziz University for Health Sciences.

Objetivos O ozônio tem sido usado como um método alternativo para a descontaminação de água, alimentos, equipamentos e instrumentos. O objetivo deste estudo foi avaliar os efeitos antimicrobianos da água ozonizada na higienização de instrumentos odontológicos contaminados por Escherichia coli , Staphylococcus aureus , Candida albicans e esporos de Bacillus atrophaeus . Métodos 3 min. O grupo controle foi composto por amostras expostas à água destilada por 30 minutos. Após a exposição à água ozonizada, alíquotas de 0,1 mL foram semeadas em ágar BHI para contar as unidades formadoras de colônias por mililitro (UFC / mL) de E. coli , S. aureus e B. atrophaeus. O ágar Sabouraud dextrose foi utilizado para contagem da UFC / mL de C. albicans . Os resultados foram submetidos à análise de variância e ao teste de Tukey. Resultados log) seguido por S. aureus (2.14–3.19 log), C. albicans (1.44–2.14 log) e os esporos de B. atrophaeus (1.01–1.98 log). Conclusão A água ozonizada foi eficaz na redução da UFC dos esporos de E. coli , S. aureus , C. albicans e B. atrophaeus , sugerindo que a água ozonizada pode ser utilizada na higienização de instrumentos dentários.