Please use this identifier to cite or link to this item: http://repositorio.unitau.br/jspui/handle/20.500.11874/1550
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dc.contributor.authorKanamura, Herminia Ypt_BR
dc.contributor.authorHancock, Kathypt_BR
dc.contributor.authorRodrigues, Vanderleipt_BR
dc.contributor.authorDamian, Raymond Tpt_BR
dc.date.accessioned2019-09-11T20:58:42Z-
dc.date.available2019-09-11T20:58:42Z-
dc.date.issued2002-
dc.citation.volume97pt_BR
dc.citation.issue5pt_BR
dc.citation.spage711-
dc.citation.epage716-
dc.identifier.doi10.1590/S0074-02762002000500022pt_BR
dc.identifier.issn1678-8060-
dc.identifier.urihttp://repositorio.unitau.br/jspui/handle/20.500.11874/1550-
dc.description.abstractA study was undertaken to search for DNA recombinant Schistosoma mansoni proteins responsible for eliciting an antibody response from the host at a very early phase after infection. A S. mansoni adult worm cDNA expression library was screened using pooled sera from baboons with four weeks of infection. Based on their specific reactivity with the S. mansoni infected sera and no reactivity when tested against the pre-infection sera from the same baboons, four clones were selected for further studies. Sequence analysis revealed that they were homologous to the S. mansoni heat shock protein 70 (hsp70). The insert sizes of the four selected clones varied from 1150 to 2006 bp. The preliminary characterization for antibody reactivity against a panel of baboon sera showed that the longest clone was the most reactive, eight out of eight acute and three out of four chronic sera reacting positively to this clone. The shortest clone was the least reactive. Our results suggest that the S. mansoni hsp70 elicits an early and strong antibody response in baboons and that antibodies to this protein can be detected in chronically infected animals. Therefore S. mansoni hsp70 may be a valid target for immunodiagnosis. However further studies are needed to identify the portion of the hsp70 that best fits the requirements for a valuable diagnostic antigen.en
dc.description.provenanceMade available in DSpace on 2019-09-11T20:58:42Z (GMT). No. of bitstreams: 0 Previous issue date: 2002en
dc.languageInglêspt_BR
dc.publisherInstituto Oswaldo Cruz, Ministério da Saúde-
dc.publisher.countryBrasilpt_BR
dc.relation.ispartofMemórias do Instituto Oswaldo Cruz-
dc.rightsAcesso Abertopt_BR
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0*
dc.sourceScielopt_BR
dc.subject.otherSchistosoma mansonien
dc.subject.othercDNAen
dc.subject.otherheat shock protein 70en
dc.subject.otherantibody responseen
dc.subject.otherbaboonen
dc.titleSchistosoma mansoni heat shock protein 70 elicits an early humoral immune response in S. mansoni infected baboonsen
dc.typeArtigo de Periódicopt_BR
dc.contributor.researcheridRodrigues, Vanderlei/I-4528-2012pt_BR
dc.description.affiliation[Kanamura, Herminia Y] Universidade de São Paulo, Brazil-
dc.description.affiliationHancock, Kathy] Centers for Diseases Control and Prevention, USA-
dc.description.affiliationKanamura, Herminia Y] Universidade de Taubaté, Brasil-
dc.description.affiliationRodrigues, Vanderlei] Universidade de São Paulo, Brazil-
dc.description.affiliationDamian, Raymond T] University of Georgia, USA-
dc.subject.researchareaParasitologyen
dc.subject.researchareaTropical Medicineen
dc.subject.scieloareaParasitologyen
dc.subject.scieloareaTropical Medicineen
dc.identifier.scieloSCIELO:S0074-02762002000500022-
Appears in Collections:Artigos de Periódicos

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