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DC Field | Value | Language |
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dc.contributor.author | Saad, Wilson Abrao | pt_BR |
dc.contributor.author | Guarda, Ismael Francisco Motta Siqueira | pt_BR |
dc.contributor.author | Camargo, Luiz Antonio de Arruda | pt_BR |
dc.contributor.author | Santos, Talmir Augusto Faria Brizola dos | pt_BR |
dc.contributor.author | Simões, Sylvio | pt_BR |
dc.contributor.author | Saad, William Abrao | pt_BR |
dc.date.accessioned | 2019-09-12T16:26:07Z | - |
dc.date.available | 2019-09-12T16:26:07Z | - |
dc.date.issued | 2006 | - |
dc.citation.volume | 6 | pt_BR |
dc.citation.issue | 4 | pt_BR |
dc.citation.spage | 597 | - |
dc.citation.epage | 602 | - |
dc.identifier.doi | 10.3923/jms.2006.597.602 | pt_BR |
dc.identifier.issn | 16824474 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-33750375543&doi=10.3923%2fjms.2006.597.602&partnerID=40&md5=4509eed008e13755ce4f4a7fdfe7f560 | - |
dc.identifier.uri | http://repositorio.unitau.br/jspui/handle/20.500.11874/1873 | - |
dc.description.abstract | The median preoptic nucleus (MnPO) is one of most important site of the lamina terminalis implicated in the regulation of hydro electrolytic and cardiovascular balance. The purpose of this study was to determine the effect of L-Type calcium channel antagonist, nifedipine, on the increase of median arterial blood pressure (MAP) induce by angiotensin II (ANG II) injected into the MnPO. The influence of nitric oxide (NO) on nifedipine antipressor action has also been studied by utilizing NW-nitro-L-arginine methyl ester (L-NAME) (40 μg 0.2 μL-1) a NO synthase inhibitor (NOSI), 7-nitroindazole (7-NIT) (40 μg 0.2 μL-1), a specific neuronal NO synthase inhibitor (nNOSI) and sodium nitroprusside (SNP) (20 μg 0.2 μL-1) a NO donor agent. We have also investigated the central role of losartan and PD123349 (20 nmol 0.2 μL-1), AT1 and AT2, respectively (selective non peptide ANG II receptor antagonists), in the pressor effect of ANG II (25 pmol 0.2 μL-1) injected into the MnPO. Male Wistar rats weighting 200-250 g, with cannulae implanted into the MnPO were utilized. Losartan injected into the MnPO, prior to ANG II, blocked the pressor effect of ANGII. PD 123319 only decreased the pressor effect of ANG II. Rats pre-treated with either 50 μg 0.2 μL-1 or 100 μg 0.2 μL-1 of nifedipine, followed by 25 pmol 0.2 μL-1 of ANG II, decreased ANG II-pressor effect. L-NAME potentiated the pressor effect of ANG II. 7-NIT injected prior to ANG II into the MnPO also potentiated the pressor effect of ANGII but with less intensity than that of L-NAME. SNP injected prior to ANG II blocked the pressor effect of ANG II. The potentiation action of L-NAME and 7-NIT on ANG II-pressor effect was blocked by prior injection of nifedipine. The results described in this study provide evidence that calcium channels play important roles in central ANG II-induced pressor effect. The structures containing NO in the brain, such as MnPO, include both endothelial and neuronal cells, which might be responsible for the influence of nifedipine on the pressor effect of ANG II. These data have shown the functional relationship between L-Type calcium channel and a free radical gas NO in the MnPO, on the control of ANG II-induced pressor effect acting in AT1 and AT2 receptors. | en |
dc.description.abstract | O núcleo pré-óptico mediano (MnPO) é um dos locais mais importantes da lâmina terminal, implicado na regulação do equilíbrio hidroeletrolítico e cardiovascular. O objetivo deste estudo foi determinar o efeito do antagonista dos canais de cálcio do tipo L, a nifedipina, no aumento da pressão arterial média (PAM) induzida pela angiotensina II (ANG II) injetada no MnPO. A influência do óxido nítrico (NO) na ação antipressora da nifedipina também foi estudada utilizando o éster metílico de N <SUP> W </SUP> -nitro-L-arginina (L-NAME) (40 µg 0,2 µL) Um inibidor da NO sintase (NOSI), 7-nitroindazol (7-NIT) (40 μg 0,2 μL L), um inibidor neuronal específico da NO sintase (nNOSI) e nitroprussiato de sódio ( SNP) (20 × 0, 2 × L <SUP> -1 </SUP>) um agente doador de NO. Também investigamos o papel central do losartan e PD123349 (20 nmol 0,2 μL <SUP> -1 </SUP>), AT <SUB> 1 </SUB> e AT <SUB> 2 </SUB>, respectivamente (seletivos antagonistas dos receptores ANG II não peptídicos), no efeito pressor de ANG II (25 pmol 0,2 µL L -1) injetado no MnPO. Utilizaram-se ratos Wistar machos, com peso entre 200 e 250 g, com cânulas implantadas no MnPO. Losartan injetado no MnPO, antes do ANG II, bloqueou o efeito pressor do ANGII. PD 123319 apenas diminuiu o efeito pressor de ANG II. Ratos pré-tratados com 50 µg 0,2 µL <SUP> -1 </SUP> ou 100 µg 0,2 µL <SUP> -1 </SUP> de nifedipina, seguidos por 25 pmol 0,2 µL <SUP> -1 </ SUP > de ANG II, diminuição do efeito pressórico de ANG II. L-NAME potencializou o efeito pressor de ANG II. O 7-NIT injetado antes do ANG II no MnPO também potencializou o efeito pressor do ANGII, mas com menos intensidade do que o do L-NAME. O SNP injetado antes do ANG II bloqueou o efeito pressor do ANG II. A ação de potenciação de L-NAME e 7-NIT no efeito pressor de ANG II foi bloqueada por injeção prévia de nifedipina. Os resultados descritos neste estudo fornecem evidências de que os canais de cálcio desempenham papéis importantes no efeito pressórico induzido por ANG II central. As estruturas que contêm NO no cérebro, como MnPO, incluem células endoteliais e neuronais, que podem ser responsáveis pela influência da nifedipina no efeito pressor da ANG II. Esses dados demonstraram a relação funcional entre o canal de cálcio do tipo L e um gás de radical livre NO no MnPO, no controle do efeito pressor induzido por ANG II que atua em AT <SUB> 1 </SUB> | pt_BR |
dc.description.provenance | Made available in DSpace on 2019-09-12T16:26:07Z (GMT). No. of bitstreams: 0 Previous issue date: 2006 | en |
dc.language | Inglês | pt_BR |
dc.relation.ispartof | Journal of Medical Sciences | - |
dc.rights | Acesso Aberto | pt_BR |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.source | Scopus | pt_BR |
dc.subject.other | Angiotensin II | en |
dc.subject.other | Blood pressure | en |
dc.subject.other | Calcium channel | en |
dc.subject.other | MnPO | en |
dc.subject.other | Nitric oxide | en |
dc.subject.other | Rats | en |
dc.subject.other | 1 (4 dimethylamino 3 methylbenzyl) 5 diphenylacetyl 4,5,6,7 tetrahydro 1h imidazo[4,5 c]pyridine 6 carboxylic acid | en |
dc.subject.other | 7 nitroindazole | en |
dc.subject.other | angiotensin | en |
dc.subject.other | angiotensin 1 receptor | en |
dc.subject.other | angiotensin 2 receptor | en |
dc.subject.other | angiotensin 2 receptor antagonist | en |
dc.subject.other | angiotensin I | en |
dc.subject.other | calcium channel L type | en |
dc.subject.other | losartan | en |
dc.subject.other | n(g) nitroarginine methyl ester | en |
dc.subject.other | nifedipine | en |
dc.subject.other | nitric oxide | en |
dc.subject.other | nitroprusside sodium | en |
dc.subject.other | animal experiment | en |
dc.subject.other | article | en |
dc.subject.other | blood pressure measurement | en |
dc.subject.other | cardiovascular function | en |
dc.subject.other | controlled study | en |
dc.subject.other | dose response | en |
dc.subject.other | drug effect | en |
dc.subject.other | endothelium cell | en |
dc.subject.other | homeostasis | en |
dc.subject.other | male | en |
dc.subject.other | mean arterial pressure | en |
dc.subject.other | nerve cell | en |
dc.subject.other | nitrergic nerve | en |
dc.subject.other | nonhuman | en |
dc.subject.other | preoptic nucleus | en |
dc.subject.other | pressor response | en |
dc.subject.other | rat | en |
dc.title | Nitrergic pathways and L-type calcium channel of MnPO influencing cardiovascular homeostasis | en |
dc.title.alternative | Vias Nitrérgicas e Canal de Cálcio do Tipo L da MnPO que Influenciam a Homeostase Cardiovascular | pt_BR |
dc.type | Artigo de Periódico | pt_BR |
dc.description.affiliation | Saad, W.A., Basic Institute of Biosciences, UNITAU, Taubaté, SP, Brazil, Department of Exact and Natural Science, UNIARA, Araraquara, SP, Brazil, Department of Physiology and Pathology, School of Dentistry, Paulista State University, Rua Humaitá 1680, 14801-903-Araraquara, SP, Brazil, Department of Anesthesiology Clinic Hospital State of Sao Paulo, Sao Paulo, Brazil, Department of Physiology, Federal University of São Carlos, São Carlos, SP, Brazil, Department of Gastroenterology, Clinic Hospital of University of São Paulo, São Paulo, Brazil | - |
dc.description.affiliation | Guarda, I.F.M.S., Department of Anesthesiology Clinic Hospital State of Sao Paulo, Sao Paulo, Brazil | - |
dc.description.affiliation | Camargo, L.A. de A., Department of Physiology and Pathology, School of Dentistry, Paulista State University, Rua Humaitá 1680, 14801-903-Araraquara, SP, Brazil | - |
dc.description.affiliation | dos Santos, T.A.F.B., Basic Institute of Biosciences, UNITAU, Taubaté, SP, Brazil | - |
dc.description.affiliation | Simões, S., Basic Institute of Biosciences, UNITAU, Taubaté, SP, Brazil | - |
dc.description.affiliation | Saad, W.A., Basic Institute of Biosciences, UNITAU, Taubaté, SP, Brazil, Department of Exact and Natural Science, UNIARA, Araraquara, SP, Brazil, Department of Physiology and Pathology, School of Dentistry, Paulista State University, Rua Humaitá 1680, 14801-903-Araraquara, SP, Brazil, Department of Anesthesiology Clinic Hospital State of Sao Paulo, Sao Paulo, Brazil, Department of Physiology, Federal University of São Carlos, São Carlos, SP, Brazil, Department of Gastroenterology, Clinic Hospital of University of São Paulo, São Paulo, Brazil | - |
dc.identifier.scopus | 2-s2.0-33750375543 | - |
dc.contributor.scopus | 7102555761 | pt_BR |
dc.contributor.scopus | 6508355383 | pt_BR |
dc.contributor.scopus | 35963541600 | pt_BR |
dc.contributor.scopus | 35577775000 | pt_BR |
dc.contributor.scopus | 7007015021 | pt_BR |
dc.contributor.scopus | 7102555761 | pt_BR |
Appears in Collections: | Artigos de Periódicos |
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