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metadata.dc.type: | Artigo de Periódico |
Title: | Antimicrobial photodynamic therapy combined with conventional endodontic treatment to eliminate root canal biofilm infection |
Authors: | Garcez, Aguinaldo Silva Ribeiro, Martha S. Tegos, George Nunez, Silvia Cristina Jorge, Antonio Olavo Cardoso Hamblin, Michael |
Abstract: | Background and Objective: To compare the effectiveness of antimicrobial photodynamic therapy (PDT), standard endodontic treatment and the combined treatment to eliminate bacterial biofilms present in infected root canals. Study Design/Materials and Methods: Ten single-rooted freshly extracted human teeth were inoculated with stable bioluminescent Gram-negative bacteria, Proteus mirabilis and Pseudomonas aeruginosa to form 3-day biofilms in prepared root canals. Biolumineseence imaging was used to serially quantify bacterial burdens. PDT employed a conjugate between polyethylenimine and chlorin(e6) as the photosensitizer (PS) and 660-nm diode laser light delivered into the root canal via a 200-mu fiber, and this was compared and combined with standard endodontie treatment using mechanical debridement and antiseptic irrigation. Results: Endodontic therapy alone reduced bacterial bioluminescence by 90% while PDT alone reduced bioluminescence by 95%. The combination reduced bioluminescence by >98%, and importantly the bacterial regrowth observed 24 hours after treatment was much less for the combination (P < 0.0005) than for either single treatment. Conclusions: Bioluminescence imaging is an efficient way to monitor endodontic therapy. Antimicrobial PDT may have a role to play in optimized endodontic therapy. |
metadata.dc.language: | Inglês |
metadata.dc.publisher.country: | Estados Unidos |
Publisher: | Wiley |
metadata.dc.rights: | Acesso Restrito |
metadata.dc.identifier.doi: | 10.1002/lsm.20415 |
URI: | http://repositorio.unitau.br/jspui/handle/20.500.11874/2530 |
Issue Date: | 2007 |
Appears in Collections: | Artigos de Periódicos |
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