Enzymatic Activity, Sensitivity to Antifungal Drugs and Baccharis dracunculifolia Essential Oil by Candida Strains Isolated from the Oral Cavities of Breastfeeding Infants and in Their Mothers' Mouths and Nipples

Abstract

Candida strains can cause oral candidosis, as well as nipples candidosis and lead to premature weaning or yeast transmission. The aim of this study was to evaluate 51 Candida isolates obtained from the oral cavities of infants during breastfeeding and mothers' oral cavities and nipples, their enzymatic activity and their sensitivity to amphotericin B, fluconazole and Baccharis dracunculifolia essential oil. Among the studied strains, 96.1% produced phospholipase and 78.4% produced proteinase. The antifungal resistance was only observed among isolates of C. albicans, for which three strains showed a resistant activity to fluconazole and one showed a resistant activity to amphotericin B. All strains were sensitive to B. dracunculifolia essential oil with MIC between 0.2 and 6.25 mg/ml. It was concluded that most of the strains showed significant enzymatic activity and were sensitive to amphotericin B and fluconazole. B. dracunculifolia essential oil inhibited the growth of all strains, including the ones resistant to commercial antifungal agents.

As cepas de Candida podem causar candidose oral, bem como candidose nos mamilos e levar ao desmame prematuro ou à transmissão de leveduras. O objetivo deste estudo foi avaliar 51 isolados de Candida obtidos das cavidades orais de lactentes durante a amamentação e as cavidades e mamilos orais das mães, sua atividade enzimática e sua sensibilidade ao anfotericina B, fluconazol e óleo essencial de Baccharis dracunculifolia . Entre as linhagens estudadas, 96,1% produziram fosfolipase e 78,4% produziram proteinase. A resistência antifúngica foi observada apenas nos isolados de C. albicans , para os quais três linhagens apresentaram atividade resistente ao fluconazol e uma mostrou atividade resistente à anfotericina B. Todas as linhagens foram sensíveis a B. dracunculifoliaóleo essencial com CIM entre 0,2 e 6,25 mg / ml. Concluiu-se que a maioria das cepas apresentou atividade enzimática significativa e foi sensível à anfotericina B e fluconazol. O óleo essencial de B. dracunculifolia inibiu o crescimento de todas as linhagens, incluindo as resistentes a agentes antifúngicos comerciais.