Please use this identifier to cite or link to this item:
metadata.dc.type: Artigo de Periódico
Title: Schistosoma mansoni heat shock protein 70 elicits an early humoral immune response in S. mansoni infected baboons
Authors: Kanamura, Herminia Y
Hancock, Kathy
Rodrigues, Vanderlei
Damian, Raymond T
Abstract: A study was undertaken to search for DNA recombinant Schistosoma mansoni proteins responsible for eliciting an antibody response from the host at a very early phase after infection. A S. mansoni adult worm cDNA expression library was screened using pooled sera from baboons with four weeks of infection. Based on their specific reactivity with the S. mansoni infected sera and no reactivity when tested against the pre-infection sera from the same baboons, four clones were selected for further studies. Sequence analysis revealed that they were homologous to the S. mansoni heat shock protein 70 (hsp70). The insert sizes of the four selected clones varied from 1150 to 2006 bp. The preliminary characterization for antibody reactivity against a panel of baboon sera showed that the longest clone was the most reactive, eight out of eight acute and three out of four chronic sera reacting positively to this clone. The shortest clone was the least reactive. Our results suggest that the S. mansoni hsp70 elicits an early and strong antibody response in baboons and that antibodies to this protein can be detected in chronically infected animals. Therefore S. mansoni hsp70 may be a valid target for immunodiagnosis. However further studies are needed to identify the portion of the hsp70 that best fits the requirements for a valuable diagnostic antigen.
metadata.dc.language: Inglês Brasil
Publisher: Instituto Oswaldo Cruz, Ministério da Saúde
metadata.dc.rights: Acesso Aberto
metadata.dc.identifier.doi: 10.1590/S0074-02762002000500022
Issue Date: 2002
Appears in Collections:Artigos de Periódicos

Files in This Item:
There are no files associated with this item.

This item is licensed under a Creative Commons License Creative Commons