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http://repositorio.unitau.br/jspui/handle/20.500.11874/1552
metadata.dc.type: | Artigo de Periódico |
Title: | Efficacy of an enzyme-linked immunosorbent assay as a diagnostic tool for schistosomiasis mansoni in individuals with low worm burden |
Authors: | Oliveira, Edward José de Kanamura, Hermínia Yohko Lima, Dirce Mary Correia |
Abstract: | IgM-ELISA is an immunoenzymatic method useful for detection of IgM antibodies against a fraction of Schistosoma mansoni adult worm antigen (AWA) that is soluble in trichloroacetic acid (AWA-TCA). This method was applied to three groups of individuals with different clinical and epidemiological characteristics, and the results compared with those obtained by other diagnostic methods: immunofluorescence test for detection of IgM antibodies (IgM-IFT) or IgG antibodies (IgG-IFT), ELISA for detection of IgG antibodies (IgG-ELISA), and two parasitological methods, Kato-Katz and miracidium hatching. The IgM-ELISA presented a sensitivity of 98%, when the parasitologic fecal examination was defined as reference diagnostic method, and a specificity of 98 and 97.3%, respectively for the group of clinically healthy individuals and other helminth carriers. A comparative analysis between the results of IgM-ELISA and those obtained by other serologic tests showed a good degree of agreement, with Kappa indices ranging from 0.95 to 0.98. The diagnostic efficacy of 97.8%, as determined with schistosomiasis patients with low parasitic burden, suggests the excellent performance of the IgM-ELISA and its usefulness for the diagnosis of schistosomiasis when applied in low endemic areas. |
metadata.dc.language: | Inglês |
metadata.dc.publisher.country: | Brasil |
Publisher: | Instituto Oswaldo Cruz, Ministério da Saúde |
metadata.dc.rights: | Acesso Aberto |
metadata.dc.rights.uri: | https://creativecommons.org/licenses/by-nc-nd/4.0 |
metadata.dc.identifier.doi: | 10.1590/S0074-02762005000400013 |
URI: | http://repositorio.unitau.br/jspui/handle/20.500.11874/1552 |
Issue Date: | 2005 |
Appears in Collections: | Artigos de Periódicos |
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S0074-02762005000400013.pdf | 448.98 kB | Adobe PDF | View/Open |
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